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Quantitative Proteomics of Cell Factories

Development of advanced quantitive proteomics methods to uncover mechanistic biological insight into pure microbial cultures, co-cultures and microbial communities (metaproteomics). 

We have developed cross-species methods to look at unsequenced organisms and then applied this to metaproteomics of natural aquatic microbial assemblages.


Published Work

Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources


  • E Hounslow, C A Evans, J Pandhal, T Sydney, N Couto, T K Pham, D James Gilmour, P C Wright. Biotechnol Biofuels 14(1):121

Metabolic Insights Into Infochemicals Induced Colony Formation and Flocculation in Scenedesmus subspicatus Unraveled by Quantitative Proteomics


  • Sebastiana Roccuzzo, Narciso Couto, Esther Karunakaran, Rahul Vijay Kapoore, Thomas O Butler, Joy Mukherjee, Erika M Hansson, Andrew P Beckerman, Jagroop Pandhal. Front Microbiol 7;11:792

Quantitative proteomics reveals reduction in photosynthetic capacity of a B12-dependent alga grown in mutualistic coculture with bacteria


  • Helliwell KE, Pandhal J, Cooper MB, Longworth J, Kudahl UJ, Russo D, Tomsett EV, Bunbury F, Salmon D, Wright PC, Smirnoff N, Smith AG (2018) New Phytologist 217(2):599-612.

A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment


  • Russo D, Couto N, Beckerman AP, Pandhal J, (2016) Frontiers in Microbiology 7.

Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line


  • Chiverton LM, Evans C, Pandhal J, Landels AR, Rees BJ, Levison PR, Wright PC, Smales CM. (2016) Biotechnol J. 11(8):1014-24

HILIC- and SCX-based quantitative proteomics of Chlamydomonas reinhardtii during nitrogen starvation induced lipid and carbohydrate accumulation


  • Longworth J, Noirel J, Pandhal J, Wright P.C, Vaidyanathan S. (2012) Journal of Proteome Research 11(12):5959-71.

An insight into iTRAQ: Where do we stand now?


  • Evans C., Noirel J., Ow S.Y., Salim M., Pereira-Medrano AG., Pham T.K., Couto N., Pandhal J., Zou X., Karunakaran E., Biggs C.A., Wright P.C. (2012) Analytical and Bioanalytical Chemistry 404(4):1011-27.

Methods in quantitative proteomics: Setting iTRAQ on the right track


  • Noirel J., Evans C., Salim M., Mukherjee J., Ow S.Y., Pandhal J., Pham K., Biggs C.A., Wright P.C. (2011) Current Proteomics 8(1):17-30.

A systems biology approach to investigate the response of Synechocystis sp. PCC6803 to a high salt environment


  • Pandhal J., Noirel J., Wright P.C., Biggs C.A. (2009) Saline Systems, 7;5:8. 

Comparative proteomics study of salt tolerance between a nonsequenced extremely halotolerant cyanobacterium and its mildly halotolerant relative using in vivo metabolic labeling and in vitro isobaric labelling


  • Pandhal J., Ow S.Y., Wright P.C., Biggs C.A. (2009)  Journal of Proteome Research, (2):818-28. 

A cross-species quantitative proteomic study of salt adaptation in a halotolerant environmental isolate using 15N metabolic labelling


  • Pandhal J., Snijders A.P., Wright P.C., Biggs C.A. (2008) Proteomics (11):2266-84. 

Proteomics with a pinch of salt: A cyanobacterial perspective


  • Pandhal J., Wright P.C., Biggs C.A. (2008) Saline Systems, 4:1. 

A Quantitative Proteomic Analysis of Light Adaptation in a Globally Significant Marine Cyanobacterium Prochlorococcus marinus MED4


  • Pandhal, J., Wright, P.C., and Biggs, C.A. (2007) Journal of  Proteome Research, 6, 3, 996 – 1005.

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