By Chandrea Ramalingam
My PhD project is funded by White Rose BBSRC DTP and Haleon (formerly GSK) in Mechanistic Biology, which aims to study oral microbial communities through nanopore sequencing, biochemical virulence assays, and to understand their functional response we have chosen metaproteomics. Using chlorhexidine (CHX) and other novel compounds in varying concentrations, we also aim to understand the role of the human oral microbiome in promoting anti-microbial resistance. So far, we have shown that we can recreate complex oral microbial communities in vitro and tested the effect of CHX. Future work will include studying the functional response of the two biofilm models using metaproteomics. Thats why we collaborate with Jags Pandhal and his group- microbial communities and their function are our window into the impact of novel compounds. Microbial communities structure might not change that much, but what about their functional responses? Improving our understanding of how the oral microbiome reacts to routine antimicrobial use is key to future development of actives.
LIVE/DEAD STAINING OF MIXED-SPECIES BIOFILM
NANOPORE SEQUENCING OF SALIVA AND MIXED-SPECIES BIOFILM SHOWING THE TAXONOMICAL CLASSIFICATION